Type 2 diabetes mellitus is known to increase degree of arteriosclerosis by decreasing vascular endothelial function.
In type 2 diabetic patients, habitual aerobic exercise decreases degree of arteriosclerosis by increasing the production of endothelium-derived relaxing substances (nitric oxide: NO). In recent years, long-term chlorella intake with antioxidant effects has been reported to decrease degree of arteriosclerosis in healthy middle or advanced, and young people. However, it is unknown whether long-term chlorella intake improves degree of arteriosclerosis in type 2 diabetic patients and the mechanism is also unclear. In this study, we examined whether long-term chlorella intake decreases degree of arteriosclerosis in type 2 diabetic rats with increasing degree of arteriosclerosis by increasing the production of endothelium-derived relaxing substances.
OLETF male rats (type 2 diabetic model rat) aged 20 weeks were divided into 4 groups, 7 rats in each group as follows, and the test was conducted. As a healthy control group, we used LETO rats that were housed under resting conditions for the same period.
 OLETF healthy control group (LETO group)
 Resting control group (OLETF-Con group)
 Chlorella intake group (OLETF-CH group)
 Aerobic training group (OLETF-Ex group)
OLETF-CH group received basic feed containing 0.5% chlorella powder for 8 weeks. OLETF-Ex group ran on a treadmill at 25 m/min for 60 minutes 5 days a week for 8 weeks.
As an index for degree of arteriosclerosis, carotid-femoral pulse wave velocity (cpPWV) was measured.
Also, phosphorylation of endothelial NO synthase (eNOS) in arterial tissue was measured with the Western blotting method and blood NOx (nitrite/nitrate) level was measured with the Griess method.
Although cfPWV in OLETF-Con group significantly increased as compared with that in LETO group, cfPWV in OLETF-CH and OLETF-Ex groups significantly decreased as compared with that in OLETF-Con group (P<0.05). (Fig. 1) Also, phosphorylation of eNOS and blood NOx level in OLETF-Con group significantly decreased as compared with those in LETO group, whereas they significantly increased in OLETF-Ex and OLETF-CH groups. (Figs. 2 and 3)The above results suggested that as with an aerobic training, long-term chlorella intake could have effects of decreasing degree of arteriosclerosis in type 2 diabetic rats by increasing the production of endothelium-derived relaxing substances.
Academic society：The 71st Annual Meeting of Japan Society of Nutrition and Food Science
Title：Long-Term Chlorella Intake Decreases Degree of Arteriosclerosis in Type 2 Diabetic Rats by Increasing Production of Endothelium-Derived Relaxing Substances
Authors：Shumpei Fujie, Natsuki Hasegawa, Naoki Horii, Toru Mizoguchi, Masato Onishi,Masataka Uchida, Motoyuki Iemitsu
Affiliation：1) Ritsumeikan University Graduate School of Sport and Health Science
2) Research Fellowship for Young Scientists (DC) of Japan Society for the Promotion of Science
3) Sun Chlorella Corporation